Ngaphezu kwezingozi ezivamile zokuphepha ezindaweni eziningi zokusebenza zansuku zonke (njengezingozi zikagesi nezomlilo), amalabhorethri e-cellculture anezingozi eziningi ezithile kanye nezingozi ezihlobene nokuphathwa nokusetshenziswa kwamangqamuzana omuntu noma ezilwane kanye nezicubu, kanye nobuthi, ukugqwala noma okuguquguqukayo. izincibilikisi.Ama-reagents.Izingozi ezivamile ukubhobozwa ngengozi kwezinalithi zesisirinji noma ezinye izibukhali ezingcolile, ukuchitheka nokuchaphaza esikhumbeni nolwelwesi lwamafinyila, ukungenwa ngamapayipi omlomo, nokuhogela ama-aerosol athathelwanayo.
Umgomo oyisisekelo wanoma yiluphi uhlelo lwe-biosafety ukunciphisa noma ukuqeda ukuchayeka kwezisebenzi zaselabhorethri kanye nemvelo yangaphandle kuma-agent angaba yingozi ezinto eziphilayo.Isici sokuphepha esibaluleke kakhulu kumalabhorethri we-cell culture ukuhambisana ngokuqinile nemikhuba namasu ajwayelekile e-microbiological.
1. Izinga le-Biosafety
Imithetho nezincomo zase-US mayelana nokuphepha kwemvelo ziqukethwe kumbhalo othi “Biosafety in Microbiology and Biomedical Laboratories” olungiswe yiCenters for Disease Control (CDC) kanye neNational Institutes of Health (NIH) futhi yanyatheliswa isevisi yoMnyango Wezempilo wase-US.Lo mbhalo uchaza amazinga amane akhuphukayo okubamba, abizwa ngokuthi amazinga 1 kuya ku-4 we-biosafety, futhi uchaza izinqubo ze-microbiological, okokusebenza kokuphepha, kanye nezinyathelo zokuvikela indawo zamazinga engozi ahambisanayo ahlobene nokuphatha amagciwane athile.
1.1 I-Biosafety Level 1 (BSL-1)
I-BSL-1 iyizinga eliyisisekelo lokuvikela elivamile ezindaweni eziningi zokucwaninga naselabhorethri zomtholampilo, futhi ifanele ama-reagents aziwa ngokuthi awabangeli isifo kubantu abavamile nabanempilo.
1.2 I-Biosafety Level 2 (BSL-2)
I-BSL-2 ifanele izidakamizwa ezinobungozi obumaphakathi ezaziwa ngokubangela izifo zabantu zobunzima obuhlukile ngokumunca noma ngokuchayeka kwe-transdermal noma mucosal.Amalabhorethri amaningi e-cellculture kufanele azuze okungenani i-BSL-2, kodwa izidingo ezithile zincike kumugqa weseli osetshenzisiwe kanye nohlobo lomsebenzi owenziwe.
1.3 I-Biosafety Level 3 (BSL-3)
I-BSL-3 ifanele amagciwane omdabu noma angaphandle anamandla aziwayo okudluliselwa kwe-aerosol, kanye namagciwane angabangela izifo ezimbi kakhulu nezinamandla okubulala.
1.4 I-Biosafety Level 4 (BSL-4)
I-BSL-4 ifanele abantu abanamagciwane angaphandle ayingozi kakhulu futhi angalashwa abangela izifo ezisongela ukuphila ngama-aerosols athathelwanayo.Lawa ma-ejenti anqunyelwe kulabhorethri evalekile kakhulu.
2. Ishidi Ledatha Yezokuphepha (SDS)
Iphepha ledatha yokuphepha (SDS), elaziwa nangokuthi i-Material Safety Data sheet (MSDS), ifomu eliqukethe ulwazi mayelana nezakhiwo zezinto ezithile.I-SDS ihlanganisa idatha ephathekayo njengendawo encibilikayo, indawo ebilayo, nephoyinti elikhanyayo, ulwazi mayelana nobuthi, ukuphinda usebenze, imiphumela yezempilo, ukugcinwa nokulahlwa kwento, kanye nezinto zokuvikela ezinconyiwe nezinqubo zokusingatha ukuvuza.
3. Izinsiza Zokuphepha
Imishini yezokuphepha kumalabhorethri we-cell culture ihlanganisa izithiyo ezinkulu, njengamakhabethe e-biosafety, iziqukathi ezivaliwe, nezinye izilawuli zobunjiniyela eziklanyelwe ukuqeda noma ukunciphisa ukuchayeka ezintweni eziyingozi, kanye nemishini yokuzivikela yomuntu siqu (i-PPE) evame ukuhlanganiswa nemishini emikhulu yokuvikela.Amakhabethe okuphepha ezinto eziphilayo (okungukuthi ama-cell culture hoods) ayizinto ezisetshenziswayo ezibaluleke kakhulu, ezingalawula ukuchaphazeka okuthelelanayo noma ama-aerosol akhiqizwa izinqubo eziningi ze-microbial futhi uvimbele isiko lamaseli akho ukuthi angangcoli.
4. Izinto Zokuzivikela Zomuntu Siqu (PPE)
Imishini yokuzivikela yomuntu siqu (i-PPE) iwumgoqo oqondile phakathi kwabantu nama-ejenti ayingozi.Kubandakanya izinto zokuzivikela komuntu siqu, njengamagilavu, amajazi kanye nezingubo zaselabhu, amakhava ezicathulo, amabhuzu, ama-respirators, izivikelo zobuso, izibuko zokuphepha noma izibuko .Ngokuvamile zisetshenziswa ngokuhambisana namakhabethe okuphepha kwebhayoloji nezinye izinto zokusebenza eziqukethe ama-reagents noma izinto ezicutshungulwayo.
Isikhathi sokuthumela: Feb-01-2023