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I-CHO Cell Line Nikeza Izinsizakalo Ezenziwe Ngokwezifiso

I-CHO Cell Line Nikeza Izinsizakalo Ezenziwe Ngokwezifiso

I-HEK293T (HEK293 transformed) ulayini wamaseli umugqa wamaseli ezinso zombungu womuntu osuselwe kumbungu womuntu ngeminyaka yawo-1970.Isetshenziswa ezindaweni ezihlukahlukene zocwaningo futhi iyithuluzi elibalulekile ocwaningweni lwesisho sezakhi zofuzo, ukwakheka kwamaprotheni kanye nomsebenzi, ukudluliselwa kwesignali, nokutholakala kwezidakamizwa.Amaseli kulula ukuwedlulisela futhi ajwayele ukusetshenziselwa ukutadisha imiphumela yokukhohlisa okuhlukahlukene kofuzo, njengokuvezwa ngokweqile noma ukudilizwa kofuzo okuhlukahlukene, ku-phenotype yeseli.Amaseli nawo asetshenziswe ezifundweni ze-stem cell biology, biology yomdlavuza, kanye ne-immunology.


Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Primary Cell Culture

I-Primary cell culture inqubo esetshenziselwa ukukhulisa nokugcina amaseli e-vitro esuka kuseli elilodwa noma iqoqo lamaseli.Le nqubo isetshenziselwa ukutadisha ukuziphatha nezici zomzimba zamaseli, futhi ingasetshenziselwa izinhlelo zokusebenza ezihlukahlukene, okuhlanganisa ukuhlolwa kwezidakamizwa, ucwaningo lwezokwelapha, kanye nokwelapha okusekelwe kumaseli.Isiko leseli eliyisisekelo lenziwa endaweni elawulwayo, ngokuvamile ebhentshini laselabhorethri, futhi lisekelwa uhla lwemishini ekhethekile nama-reagents.Amangqamuzana agcinwa ephila ngokunikeza imisoco edingekayo nokugcina izinga lokushisa elifanele, i-pH, namazinga omoya-mpilo.Amaseli aphinde agadwe noma yiziphi izimpawu zokuxineka noma ukungcola, futhi isiko lihlale lihlolwa ukuze kutholakale noma yiziphi izinguquko ekukhuleni noma ekumisweni kokwakheka kwazo.

sebenzela1

I-Pro-antibody Design Platform enikwe amandla yi-AI

I-AlfaCap™

sebenzela2

I-AI enikwe amandla i-Site-Specific Integration Cell Line Development Platform

sebenzela3

I-Al-enabled Cell Culture Media Development Platform

Ingqamuzana Lomuntu

Ingqamuzana lomuntu liyingxenye eyisisekelo yokuphila.Umzimba womuntu wakhiwe ngezigidigidi zamangqamuzana ngalinye linesakhiwo nomsebenzi ohlukile.Amangqamuzana ayizingqimba zazo zonke izinto eziphilayo futhi anesibopho sokukhula, umzimba, nezinye izinqubo ezibalulekile.Amaseli akhiwe izingxenye ezihlukahlukene, okuhlanganisa amaprotheni, i-DNA, ama-carbohydrate, ama-lipids, nama-organelles.

Secondary Cell Culture

I-secondary cell culture inqubo yokukhulisa amaseli ahlukanisiwe futhi akhule elabhorethri ngaphambili.Amangqamuzana angakhuliswa ngokufakwa kwezicubu, ahlukaniswe nama-enzyme, noma ahlanganiswe namangqamuzana awodwa.Isiko leseli yesibili lisetshenziselwa ukwandisa imigqa yamaseli, ukufunda ukuziphatha kwamaseli, nokuthuthukisa ukuhlola okusekelwe kumaseli.Izinhlobo zamaseli ezivamile ezisetshenziswa kusiko lamaseli esibili zihlanganisa ama-fibroblasts, amaseli e-endothelial, namaseli emisipha abushelelezi.


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