1.Ukukhetha umugqa weseli olungile
Lapho ukhetha ulayini weseli ofanele wokuhlolwa kwakho, sicela ucabangele le mibandela elandelayo:
a.Izinhlobo: Olayini bamaseli okungebona abantu nabangewona owezilwane ngokuvamile banemikhawulo embalwa ye-biosafety, kodwa ekugcineni ukuhlola kwakho kuzonquma ukuthi kufanele kusetshenziswe isiko lohlobo oluthile yini.
b.Izici: Iyini inhloso yocwaningo lwakho?Isibonelo, imigqa yamaseli etholakala esibindini nasezinso ingase ifaneleke kakhulu ukuhlolwa kobuthi.
c.Kunomkhawulo noma okuqhubekayo: Nakuba ukukhetha kulayini weseli enomkhawulo kungase kukunikeze izinketho ezengeziwe zokuveza umsebenzi olungile, imigqa yamaseli eqhubekayo ngokuvamile kulula ukuyihlanganisa nokuyinakekela.
d.Okuvamile noma okuguquliwe: Imigqa yamaseli aguquliwe ngokuvamile inezinga eliphezulu lokukhula kanye nokusebenza kahle kwembewu ephezulu, iyaqhubeka, futhi idinga i-serum encane endaweni yesiko, kodwa i-phenotype yabo ibe nezinguquko ezingapheli ngokuguqulwa kofuzo.
e.Izimo zokukhula nezici: Yiziphi izidingo zakho zesivinini sokukhula, ukuminyana kokugcwala kwendawo, ukusebenza kahle kwe-cloning kanye nekhono lokukhula kokumiswa?Isibonelo, ukuze uveze amaprotheni ahlanganisiwe ngesivuno esiphezulu, kungase kudingeke ukhethe imigqa yamaseli enamazinga okukhula okusheshayo kanye nekhono lokukhula ngokumiswa.
f.Ezinye izindlela: Uma usebenzisa ulayini weselula olinganiselwe, ingabe sikhona isitoko esanele esitholakalayo?Ingabe ulayini weseli ubhalwe ngokugcwele, noma kufanele uwuqinisekise ngokwakho?Uma usebenzisa ulayini weseli ongavamile, ingabe ukhona umugqa weseli olinganayo ongasetshenziswa njengesilawuli?Ingabe ulayini weseli uzinzile?Uma kungenjalo, kulula kangakanani ukukuhlanganisa nokwenza isitoko esanele esiqandisiwe sokuhlola kwakho?
2.Thola imigqa yamaseli
Ungazakhela owakho isiko kuma-primary cell, noma ungakhetha ukuthenga amasiko amaseli asunguliwe kubahlinzeki bezentengiselwano noma abangenzi nzuzo (okungukuthi amabhange amaselula).Abahlinzeki abahlonishwayo bahlinzeka ngolayini bamaseli bekhwalithi ephezulu abahlolwe ngokucophelela ukuze bathole ubuqotho futhi baqinisekise ukuthi isiko alinakho ukungcola.Sincoma ukuthi singaboleki amasiko kwamanye amalabhorethri ngoba asengozini enkulu yokungcoliswa kwesiko lamaseli.Kungakhathalekile ukuthi ikuphi, sicela uqiniseke ukuthi wonke amaseli amasha ahlolelwe ukungcola kwe-mycoplasma ngaphambi kokuthi uqale ukuyisebenzisa.
Isikhathi sokuthumela: Feb-01-2023